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Nutritional Requirements of Cells

It is essential for any bacteria to locate the appropriate environment , in which all of the substances required for energy generation and cellular biosynthesis are provided. In a laboratory or industrial fermentation settings, bacteria are grown in culture media, which is designed to provide all the essential nutrients in solution for bacterial growth: the biochemical environment. The manner, and purpose, of the culture varies widely. For example, most pathogenic bacteria of animals, which have adapted themselves to growth in animal tissues, require complex media for their growth. For many bacteria, identification can be done in the environment through inspection or using genetic techniques, however attempts to isolate and grow them in artificial culture can be difficult, and sometimes unsuccessful.

BlueWave Peptides + Nucleotides have proven to be excellent sources of Nitrogen rich nutrients to enhance population growth/density in culture media.

For many bacteria, e.g. L. bacillus, the nutritional requirements are revealed by the cell’s elemental composition: C, H, O, N, S. P, K, Mg, Fe, Ca, Mn, and traces of Zn, Co, Cu, and Mo.

In order to grow in a laboratory, these elements must be provided, as well as optimum temperature, pH, O2 concentration and other physical conditions.

Growth factors:

Growth factors are required in small amounts by bacteria because they fulfill specific roles in biosynthesis and so must be added to the medium. Growth factors are organized into three categories:

Nucleotides, Vitamins and Peptides (Amino Acids).

Fermentation Media

The requirements, both physical (temp., 02 concentration, pH…) and chemical conditions are different for different bacteria species.

Lactobacillus delbrueckii is a representative of a large group of lactic acid bacteria commonly used for assay of vitamins and amino acids. Previous nutritional investigations with this organism it to require pyridoxamine phosphate, thymidine, and an unsaturated fatty acid (e.g., oleic acid) for growth in a medium.

Essential Amino Acid
Methionine & Cystine
Phenylalanine & tyrosine

Small amounts of histidine peptides (e.g., carnosine) were shown to  duplicate the growth promoting action of enzymatic protein digests for this organism.

Omission of any one of these amino acids from the basic medium prevents growth, even after prolonged incubation. Different combinations of these amino acids is dependent on the species.